Year : 2017 | Volume
: 45 | Issue : 3 | Page : 122--128
Role of autophagy and oxidative stress in experimental diabetes in rats
Asmaa H Okasha, Hanaa H Gaballah, Soha S Zakaria, Salwa M Elmeligy
Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, Egypt
Background Diabetes mellitus (DM) is a chronic metabolic disease characterized by hyperglycemia owing to defects in insulin secretion, insulin action, or both. The exact pathogenesis of β-cell failure in type 2 DM is still unclear. However, autophagy and oxidative stress have emerged presently as major contributing factors in the development of type 2 diabetes and β-cell failure.
Aim The aim is to assess the levels of beclin-1 (autophagy regulator) and some oxidative stress markers in animal models of high-fat diet-streptozotocin-induced diabetes and to study the effect of 4-phenylbutyrate (4-PBA) on them.
Materials and methods The study was conducted on 60 male albino rats that were divided randomly into three equal groups: group I (control group), group II (diabetic group), and group III (diabetic rats treated with 4-PBA). Pancreatic tissue levels of beclin-1 were measured by enzyme-linked immuosorbent assay, whereas pancreatic tissue levels of reduced glutathione (GSH) and plasma levels of advanced oxidation protein products (AOPPs) and glucose were measured spectrophotometrically.
Results The levels of beclin-1, AOPPs, and glucose were significantly increased in diabetic group compared with control group, whereas the levels of GSH were significantly decreased. Moreover, 4-PBA significantly decreased the levels of beclin-1, AOPPs, and glucose, whereas it increased GSH levels.
Conclusion Autophagy and oxidative stress are involved in the pathogenesis of type 2 DM. Moreover, 4-PBA (a chemical chaperone) attenuated autophagy and exhibited antioxidative stress effects in rats with high-fat diet-streptozotocin-induced diabetes.
Asmaa H Okasha
Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Al-Geish Street, Tanta, 31527
|How to cite this article:|
Okasha AH, Gaballah HH, Zakaria SS, Elmeligy SM. Role of autophagy and oxidative stress in experimental diabetes in rats.Tanta Med J 2017;45:122-128
|How to cite this URL:|
Okasha AH, Gaballah HH, Zakaria SS, Elmeligy SM. Role of autophagy and oxidative stress in experimental diabetes in rats. Tanta Med J [serial online] 2017 [cited 2018 Oct 15 ];45:122-128
Available from: http://www.tdj.eg.net/article.asp?issn=1110-1415;year=2017;volume=45;issue=3;spage=122;epage=128;aulast=Okasha;type=0